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Title: Polyamine oxidase 5 loss-of-function mutations in Arabidopsis thaliana trigger metabolic and transcriptional reprogramming and promote salt stress tolerance
Authors: Zarza, Xavier 
Atanasov, Kostadin E. 
Marco, Francisco 
Arbona, Vicent 
Carrasco, Pedro 
Kopka, Joachim 
Fotopoulos, Vasileios 
Munnik, Teun 
Gómez-Cadenas, Aurelio 
Tiburcio, Antonio F. 
Alcázar, Rubén 
Keywords: Jasmonates;Polyamines;Metabolomics;Salt tolerance;Thermospermine
Category: Agriculture Forestry and Fisheries
Field: Agricultural Sciences
Issue Date: Apr-2017
Publisher: Blackwell Publishing Ltd
Source: Plant, Cell and Environment, 2017, Volume 40, Issue 4, Pages 527-542
Abstract: The family of polyamine oxidases (PAO) in Arabidopsis (AtPAO1-5) mediates polyamine (PA) back-conversion, which reverses the PA biosynthetic pathway from spermine and its structural isomer thermospermine (tSpm) into spermidine and then putrescine. Here, we have studied the involvement of PA back-conversion in Arabidopsis salinity tolerance. AtPAO5 is the Arabidopsis PAO gene member most transcriptionally induced by salt stress. Two independent loss-of-function mutants (atpao5-2 and atpao5-3) were found to exhibit constitutively higher tSpm levels, with associated increased salt tolerance. Using global transcriptional and metabolomic analyses, the underlying mechanisms were studied. Stimulation of abscisic acid and jasmonate (JA) biosynthesis and accumulation of important compatible solutes, such as sugars, polyols and proline, as well as TCA cycle intermediates were observed in atpao5 mutants under salt stress. Expression analyses indicate that tSpm modulates the transcript levels of several target genes, including many involved in the biosynthesis and signalling of JA, some of which are already known to promote salinity tolerance. Transcriptional modulation by tSpm is isomer-dependent, thus demonstrating the specificity of this response. Overall, we conclude that tSpm triggers metabolic and transcriptional reprogramming that promotes salt stress tolerance in Arabidopsis.
ISSN: 01407791
Rights: © 2016 John Wiley & Sons Ltd.
Type: Article
Appears in Collections:Άρθρα/Articles

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