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dc.contributor.authorBotsaris, George-
dc.contributor.authorSwift, Benjamin M. C.-
dc.contributor.authorSlana, Iva-
dc.contributor.authorLiapi, Maria-
dc.contributor.authorChristodoulou, Maritsa-
dc.contributor.authorHatzitofi, Maria-
dc.contributor.authorChristodoulou, Vasiliki-
dc.contributor.authorRees, Catherine-
dc.contributor.otherΜπότσαρης, Γιώργος-
dc.contributor.otherΧριστοδούλου, Μαρίτσα-
dc.contributor.otherΧατζηττοφή, Μαρία-
dc.identifier.citationInternational Journal of Food Microbiology, January 04 2016, Volume 216, Pages 91-94en_US
dc.description.abstractSurveys from different parts of the world have reported that viable Mycobacterium avium subsp. paratuberculosis (MAP) can be cultured from approximately 2% of samples of retail pasteurised milk samples. Pasteurised milk is used for the production of powdered infant formula (PIF) and therefore there is a concern that MAP may also be present in these products. Several studies have previously reported the detection of MAP in PIF using PCR-based assays. However, culture-based surveys of PIF have not detected viable MAP. Here we describe a phage amplification assay coupled with PCR (page-PCR) that can rapidly detect viable MAP in PIF. The results of a small survey showed that the phage-PCR assay detected viable MAP in 13% (4/32) of PIF samples. Culture detected viable MAP in 9% (3/32) PIF samples, all of which were also phage-PCR positive. Direct IS. 900 PCR detected MAP DNA in 22% (7/32) of PIF samples. The presence of viable MAP in PIF indicates that MAP either survived PIF manufacturing or that post-production contamination occurred. Irrespective of the route of MAP contamination, the presence of viable MAP in PIF is a potential public health concern.en_US
dc.publisherElsevier Ltden_US
dc.rights© 2015 Elsevier B.V.en_US
dc.subjectPasteurised milken_US
dc.subjectPowdered infant formulaen_US
dc.subjectRapid methodsen_US
dc.titleDetection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by cultureen_US
dc.collaborationCyprus University of Technologyen_US
dc.collaborationUniversity of Nottinghamen_US
dc.collaborationVeterinary Research Instituteen_US
dc.collaborationCyprus Veterinary Servicesen_US
dc.subject.categoryAgricultural Biotechnologyen_US
dc.journalsSubscription Journalen_US
dc.countryUnited Kingdomen_US
dc.countryCzech Republicen_US
dc.subject.fieldAgricultural Sciencesen_US
dc.publicationPeer Revieweden_US
item.fulltextWith Fulltext-
item.languageiso639-1other- of Agricultural Sciences, Biotechnology and Food Science- of Geotechnical Sciences and Environmental Management- of Geotechnical Sciences and Environmental Management-
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