Please use this identifier to cite or link to this item:
Title: Detection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by culture
Authors: Botsaris, George 
Swift, Benjamin M. C. 
Slana, Iva 
Liapi, Maria 
Christodoulou, Maritsa 
Hatzitofi, Maria 
Christodoulou, Vasiliki 
Rees, Catherine 
Keywords: PCR;Paratuberculosis;Pasteurised milk;Phage;Powdered infant formula;Rapid methods
Category: Agricultural Biotechnology
Field: Agricultural Sciences
Issue Date: 4-Jan-2016
Publisher: Elsevier Ltd
Source: International Journal of Food Microbiology, January 04 2016, Volume 216, Pages 91-94
Abstract: Surveys from different parts of the world have reported that viable Mycobacterium avium subsp. paratuberculosis (MAP) can be cultured from approximately 2% of samples of retail pasteurised milk samples. Pasteurised milk is used for the production of powdered infant formula (PIF) and therefore there is a concern that MAP may also be present in these products. Several studies have previously reported the detection of MAP in PIF using PCR-based assays. However, culture-based surveys of PIF have not detected viable MAP. Here we describe a phage amplification assay coupled with PCR (page-PCR) that can rapidly detect viable MAP in PIF. The results of a small survey showed that the phage-PCR assay detected viable MAP in 13% (4/32) of PIF samples. Culture detected viable MAP in 9% (3/32) PIF samples, all of which were also phage-PCR positive. Direct IS. 900 PCR detected MAP DNA in 22% (7/32) of PIF samples. The presence of viable MAP in PIF indicates that MAP either survived PIF manufacturing or that post-production contamination occurred. Irrespective of the route of MAP contamination, the presence of viable MAP in PIF is a potential public health concern.
ISSN: 01681605
Rights: © 2015 Elsevier B.V.
Type: Article
Appears in Collections:Άρθρα/Articles

Files in This Item:
File Description SizeFormat
Botsaris.pdfThis is a RoMEO green journal250.6 kBAdobe PDFView/Open
Show full item record

Page view(s)

Last Week
Last month
checked on Sep 19, 2019


checked on Sep 19, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.