Please use this identifier to cite or link to this item: https://ktisis.cut.ac.cy/handle/10488/8151
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dc.contributor.authorFilippou, Panagiota S.-
dc.contributor.authorFotopoulos, Vasileios-
dc.contributor.authorAntoniou, Chrystalla-
dc.contributor.otherΦιλίππου, Παναγιώτα-
dc.contributor.otherΦωτόπουλος, Βασίλειος-
dc.contributor.otherΑντωνίου, Χρυστάλλα-
dc.date.accessioned2013-04-26T09:04:18Zen
dc.date.accessioned2013-05-17T07:12:45Z-
dc.date.accessioned2015-12-02T14:26:34Z-
dc.date.available2013-04-26T09:04:18Zen
dc.date.available2013-05-17T07:12:45Z-
dc.date.available2015-12-02T14:26:34Z-
dc.date.issued2013-03-
dc.identifier.citationFree Radical Biology and Medicine, Volume 56, March 2013, Pages 172-183en_US
dc.identifier.issn08915849-
dc.description.abstractNitric oxide (NO), polyamines, and proline have all been suggested to play key roles in a wide spectrum of physiological processes and abiotic stress responses. Although exogenous application of polyamines has been shown to induce NO production, the effect of NO on polyamine biosynthesis has not yet been elucidated. Several reports exist that demonstrate the protective action of sodium nitroprusside (SNP), a widely used NO donor, which acts as a signal molecule in plants responsible for the regulation of the expression of many defense-related enzymes. This study attempted to provide a novel insight into the effects of application of low (100 μΜ) and high (2.5 mM) concentrations of SNP on the biosynthesis of two major abiotic stress response-related metabolites, polyamines and proline, in mature (40 day) and senescing (65 day) Medicago truncatula plants. Physiological data showed that long-term (24 h), higher SNP concentration resulted in decreased photosynthetic rate and stomatal conductance followed by intracellular putrescine and proline accumulation, as a result of an increase in biosynthetic arginine decarboxylase (ADC) and Δ1 -pyrroline-5-carboxylate synthetase (P5CS) enzymatic activity, respectively. Further analysis of polyamine oxidase (PAO)/diamine oxidase (DAO) polyamine catabolic enzymes indicated that DAO enzymatic activity increased significantly in correlation with putrescine accumulation, whereas PAO activity, involved in spermidine/spermine degradation, increased slightly. Moreover, transcriptional analysis of polyamine and proline metabolism genes (P5CS, P5CR, ADC, SPMS, SPDS, SAMDC, PAO, DAO) further supported the obtained data and revealed a complex SNP concentration-, time-, and developmental stage-dependent mechanism controlling endogenous proline and polyamine metabolite production. This is the first report to provide a global analysis leading to a better understanding of the role of the widely used NO donor SNP in the regulation of key stress-related metabolic pathways.en_US
dc.formatpdfen_US
dc.language.isoenen_US
dc.publisherElsevieren
dc.relation.ispartofFree Radical Biology and Medicineen_US
dc.rightsCopyright © 2012 Elsevier Inc. All rights reserved.en_US
dc.subjectSodium nitroferricyanideen_US
dc.subjectPolyaminesen_US
dc.subjectProlineen_US
dc.subjectMedicagoen_US
dc.subjectFree radicalsen_US
dc.titleThe nitric oxide donor sodium nitroprusside regulates polyamine and proline metabolism in leaves of Medicago truncatula plantsen_US
dc.typeArticleen_US
dc.collaborationCyprus University of Technologyen_US
dc.subject.categoryBiological Sciencesen_US
dc.journalsSubscription Journalen_US
dc.reviewpeer reviewed-
dc.countryCyprusen_US
dc.subject.fieldNatural Sciencesen_US
dc.publicationPeer Revieweden_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.freeradbiomed.2012.09.037en_US
dc.dept.handle123456789/70en
cut.common.academicyear2012-2013en_US
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.deptDepartment of Multimedia and Graphic Arts-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.facultyFaculty of Fine and Applied Arts-
crisitem.author.orcid0000-0003-1205-2070-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.parentorgFaculty of Fine and Applied Arts-
crisitem.journal.journalissn08915849-
crisitem.journal.publisherElsevier-
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