Please use this identifier to cite or link to this item: https://ktisis.cut.ac.cy/handle/10488/7343
Title: Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
Authors: Li, Chunqiang 
Pastila, Riikka K. 
Pitsillides, Costas 
Major Field of Science: Agricultural Sciences
Field Category: Other Agricultural Sciences
Keywords: Harmonic generation;Collagen;Generation microscopy
Issue Date: 18-Jan-2010
Source: Optics express, 2010, vol. 18, no. 2, pp. 988-999
Volume: 18
Issue: 2
Start page: 988
End page: 999
Journal: Optics Express 
Abstract: We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact dynamically with the dermal vascular endothelium. Inflammation significantly enhances leukocyte rolling, adhesion, and tissue infiltration. After exiting the vasculature, leukocytes continue to move actively in tissue as observed by time-lapse microscopy, and are distinguishable from resident autofluorescent cells that are not motile. Because the new method alleviates the need to introduce exogenous labels, it is potentially applicable for tracking leukocytes and monitoring inflammatory cellular reactions in humans
ISSN: 1094-4087
DOI: 10.1364/OE.18.000988
Rights: © Optical Society of America
Type: Article
Affiliation: Massachusetts General Hospital 
Affiliation : Harvard Medical School 
Boston University 
Universite Laval 
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