Please use this identifier to cite or link to this item: https://ktisis.cut.ac.cy/handle/10488/7343
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dc.contributor.authorLi, Chunqiangen
dc.contributor.authorPastila, Riikka K.en
dc.contributor.authorPitsillides, Costas-
dc.contributor.otherΠιτσιλλίδης, Κώστας-
dc.date.accessioned2013-02-22T13:39:04Zen
dc.date.accessioned2013-05-17T05:22:45Z-
dc.date.accessioned2015-12-02T10:11:32Z-
dc.date.available2013-02-22T13:39:04Zen
dc.date.available2013-05-17T05:22:45Z-
dc.date.available2015-12-02T10:11:32Z-
dc.date.issued2010en
dc.identifier.citationOptics express, 2010, Volume 18, Issue 2, Pages 988-999en
dc.identifier.issn1094-4087en
dc.identifier.urihttp://ktisis.cut.ac.cy/handle/10488/7343en
dc.description.abstractWe describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact dynamically with the dermal vascular endothelium. Inflammation significantly enhances leukocyte rolling, adhesion, and tissue infiltration. After exiting the vasculature, leukocytes continue to move actively in tissue as observed by time-lapse microscopy, and are distinguishable from resident autofluorescent cells that are not motile. Because the new method alleviates the need to introduce exogenous labels, it is potentially applicable for tracking leukocytes and monitoring inflammatory cellular reactions in humansen
dc.language.isoenen
dc.publisherThe Optical Societyen
dc.rights© 2010 Optical Society of Americaen
dc.subjectLeucocytesen
dc.subjectFluorescenceen
dc.subjectAmino acidsen
dc.subjectCellsen
dc.subjectMiceen
dc.subjectPhotonsen
dc.titleImaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescenceen
dc.typeArticleen
dc.affiliationMassachusetts General Hospitalen
dc.identifier.doi10.1364/OE.18.000988en
dc.identifier.pmid20173920-
dc.dept.handle123456789/54en
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.languageiso639-1other-
crisitem.author.deptDepartment of Mechanical Engineering and Materials Science and Engineering-
crisitem.author.facultyFaculty of Engineering and Technology-
crisitem.author.parentorgFaculty of Engineering and Technology-
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