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|Title:||In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response||Authors:||Fan, Zhigang
Spencer, Joel A.
Yun, Seok Hyun Andy
Lin, Charles P.
|Major Field of Science:||Natural Sciences||Field Category:||Electrical Engineering - Electronic Engineering - Information Engineering||Keywords:||Flow cytometry;Photoacoustic effect;Vivo flow||Issue Date:||Jun-2010||Source:||Nature medicine, 2010, vol. 16, no. 6, pp. 718-722||Volume:||16||Issue:||6||Start page:||718||End page:||722||Journal:||Nature medicine||Abstract:||Here we present methods to longitudinally track islet allograft–infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (Teff cells) and discrimination between natural and induced regulatory T cells (nTreg and iTreg cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish real-time cell tracking as a powerful means to probe the dynamic cellular interplay mediating immunologic rejection or transplant tolerance||ISSN:||1546-170X||DOI:||10.1038/nm.2155||Rights:||© Nature America||Type:||Article||Affiliation:||Boston University||Affiliation :||Harvard Medical School
|Appears in Collections:||Άρθρα/Articles|
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