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Title: In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response
Authors: Fan, Zhigang 
Spencer, Joel A. 
Pitsillides, Costas 
Lü, Yan 
Singh, Gurbakhshish 
Kim, Pilhan 
Yun, Seok Hyun Andy 
Toxavidis, Vasilis 
Strom, Terry 
Lin, Charles P. 
Koulmanda, Maria 
Major Field of Science: Natural Sciences
Field Category: Electrical Engineering - Electronic Engineering - Information Engineering
Keywords: Flow cytometry;Photoacoustic effect;Vivo flow
Issue Date: Jun-2010
Source: Nature medicine, 2010, vol. 16, no. 6, pp. 718-722
Volume: 16
Issue: 6
Start page: 718
End page: 722
Journal: Nature medicine 
Abstract: Here we present methods to longitudinally track islet allograft–infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (Teff cells) and discrimination between natural and induced regulatory T cells (nTreg and iTreg cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish real-time cell tracking as a powerful means to probe the dynamic cellular interplay mediating immunologic rejection or transplant tolerance
ISSN: 1546-170X
DOI: 10.1038/nm.2155
Rights: © Nature America
Type: Article
Affiliation: Boston University 
Affiliation : Harvard Medical School 
Tufts University 
Boston University 
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