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|Title:||In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response||Authors:||Fan, Zhigang
Spencer, Joel A.
|Keywords:||T cells;Mice;Animal experimentation;Monoclonal antibodies;Flow cytometry||Issue Date:||2010||Publisher:||Nature||Source:||Nature medicine, 2010, Volume 16, Issue 6, Pages 718-722||Abstract:||Here we present methods to longitudinally track islet allograft–infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (Teff cells) and discrimination between natural and induced regulatory T cells (nTreg and iTreg cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish real-time cell tracking as a powerful means to probe the dynamic cellular interplay mediating immunologic rejection or transplant tolerance||URI:||http://ktisis.cut.ac.cy/handle/10488/7338||ISSN:||1078-8956||DOI:||10.1038/nm.2155||Rights:||© 2010 Nature America, Inc. All rights reserved||Type:||Article|
|Appears in Collections:||Άρθρα/Articles|
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