Please use this identifier to cite or link to this item: https://ktisis.cut.ac.cy/handle/10488/7337
Title: Multiphoton microscopy of live tissues with ultraviolet autofluorescence
Authors: Li, Chunqiang 
Runnels, Judith M. 
Pitsillides, Costas 
Major Field of Science: Engineering and Technology
Field Category: Mechanical Engineering;Materials Engineering
Keywords: In vivo imaging;Multiphoton-excited fluorescence microscopy (MPM);Tryptophan autofluorescence;Video-rate imaging
Issue Date: May-2010
Source: IEEE journal on selected topics in quantum electronics, 2010, vol. 16, no. 3, pp. 516-523
Volume: 16
Issue: 3
Start page: 516
End page: 523
Journal: IEEE journal on selected topics in quantum electronics 
Abstract: Current research on multiphoton autofluorescence microscopy is primarily focused on imaging the signal from reduced nicotinamide adenine dinucleatide (NADH) in tissue. NADH levels in cells are useful reporters of metabolic information, as well as early indicators in precancer and cancer diagnosis. While NADH is typically imaged in the 400-500 nm spectral window, the amino acid tryptophan is the major source of tissue fluorescence in the Ultraviolet range. Here, we briefly review current progress in multiphoton autofluorescence imaging of live tissues and cells, and report our recent findings of in vivo mouse skin imaging based on multiphoton excited tryptophan autofluorescence. This new method enables noninvasive imaging of skin tissue at video-rate and allows for the visualization and identification of cellular components in the epidermis, dermis, and muscle layers. It is also possible to image through small blood vessels in the mouse skin and observe circulating leukocytes in situ
ISSN: 1558-4542
DOI: 10.1109/JSTQE.2009.2031619
Rights: © IEEE
Type: Article
Affiliation: Boston University 
Affiliation : Harvard Medical School 
Universite Laval 
Massachusetts General Hospital 
Boston University 
Wellman Center for Photomedicine 
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