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Title: Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H +-ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum
Authors: Fotopoulos, Vasileios 
Holmes, Robert 
Hall, John L. 
Williams, Lorraine E. 
Keywords: Arabidopsis thaliana;Erysiphales;Plant pathology;Plasma membrane H+-ATPase;Cell membrane;Cloning;Gene expression;Membrane proteins;Phylogeny;Enzymology
Category: AGRICULTURAL SCIENCES;Agricultural Biotechnology;Other Agricultural Sciences
Field: Agricultural Sciences
Issue Date: Jan-2006
Publisher: Elsevier
Source: Mycological Research, Volume 110, Issue 1, January 2006, Pages 28-37
Journal: Mycological Research 
Abstract: Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H +-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H +-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen
ISSN: 09537562
Rights: © The British Mycological Society
Type: Article
Appears in Collections:Άρθρα/Articles

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