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|Title:||Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H +-ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum||Authors:||Fotopoulos, Vasileios
Hall, John L.
Williams, Lorraine E.
|Keywords:||Arabidopsis thaliana;Erysiphales;Plant pathology;Plasma membrane H+-ATPase;Cell membrane;Cloning;Gene expression;Membrane proteins;Phylogeny;Enzymology||Category:||AGRICULTURAL SCIENCES;Agricultural Biotechnology;Other Agricultural Sciences||Field:||Agricultural Sciences||Issue Date:||Jan-2006||Publisher:||Elsevier||Source:||Mycological Research, Volume 110, Issue 1, January 2006, Pages 28-37||Journal:||Mycological Research||Abstract:||Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H +-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H +-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen||URI:||http://ktisis.cut.ac.cy/handle/10488/6464||ISSN:||09537562||DOI:||http://dx.doi.org/10.1016/j.mycres.2005.08.011||Rights:||© The British Mycological Society||Type:||Article|
|Appears in Collections:||Άρθρα/Articles|
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