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Title: A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus
Authors: Papayiannis, Lambros C. 
Brown, Judith K. 
Seraphides, Nicos A. 
Hadjistylli, Margarita 
Katis, Nikolaos I. 
Ioannou, Nicolas 
Major Field of Science: Agricultural Sciences
Field Category: Agricultural Biotechnology
Keywords: Bemisia tabaci complex;Haplotype;Mitochondrial COI gene;Real-time PCR;RFLP analysis
Issue Date: Dec-2009
Source: Bulletin of Entomological Research, 2009, vol. 99, no. 6, pp. 573-582
Volume: 99
Issue: 6
Start page: 573
End page: 582
Journal: Bulletin of Entomological Research 
Abstract: A real-time PCR assay based on TaqMan® technology was developed and evaluated for the rapid detection of the B and Q biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). A survey was conducted during 2005-2007 in order to identify the distribution and prevalence of B. tabaci biotypes in Cyprus using the real-time PCR assay. More than 700 adult whiteflies collected from 35 cultivated and weed plant species were individually haplotyped using TaqMan® PCR, and the results of the assay were validated by restriction fragment length polymorphism analysis and DNA sequencing of the mitochondrial cytochrome oxidase I (mtCOI) gene. Two biotypes, B and Q, were identified in the collected plant species on the island. The real-time PCR and RFLP assay consistently yielded the same results, although the real-time assay was more sensitive and less time consuming. Phylogenetic analysis of the mtCOI DNA sequences corroborated the identity of the B and Q biotypes 100% of the time and by phylogenetic analysis the haplotypes grouped, as expected, in the major North African-Mediterranean-Middle Eastern clade of the B. tabaci complex.
ISSN: 1475-2670
DOI: 10.1017/S0007485308006603
Rights: © Cambridge University Press
Type: Article
Affiliation : Agricultural Research Institute of Cyprus 
University of Arizona 
Cyprus University of Technology 
Aristotle University of Thessaloniki 
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