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|Title:||A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus||Authors:||Papayiannis, Lambros C.
Brown, Judith K.
Seraphides, Nicos A.
Katis, Nikolaos I.
|Major Field of Science:||Agricultural Sciences||Field Category:||Agricultural Biotechnology||Keywords:||Bemisia tabaci complex;Haplotype;Mitochondrial COI gene;Real-time PCR;RFLP analysis||Issue Date:||Dec-2009||Source:||Bulletin of Entomological Research, 2009, vol. 99, no. 6, pp. 573-582||Volume:||99||Issue:||6||Start page:||573||End page:||582||Journal:||Bulletin of Entomological Research||Abstract:||A real-time PCR assay based on TaqMan® technology was developed and evaluated for the rapid detection of the B and Q biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). A survey was conducted during 2005-2007 in order to identify the distribution and prevalence of B. tabaci biotypes in Cyprus using the real-time PCR assay. More than 700 adult whiteflies collected from 35 cultivated and weed plant species were individually haplotyped using TaqMan® PCR, and the results of the assay were validated by restriction fragment length polymorphism analysis and DNA sequencing of the mitochondrial cytochrome oxidase I (mtCOI) gene. Two biotypes, B and Q, were identified in the collected plant species on the island. The real-time PCR and RFLP assay consistently yielded the same results, although the real-time assay was more sensitive and less time consuming. Phylogenetic analysis of the mtCOI DNA sequences corroborated the identity of the B and Q biotypes 100% of the time and by phylogenetic analysis the haplotypes grouped, as expected, in the major North African-Mediterranean-Middle Eastern clade of the B. tabaci complex.||URI:||http://ktisis.cut.ac.cy/handle/10488/5092||ISSN:||1475-2670||DOI:||10.1017/S0007485308006603||Rights:||© Cambridge University Press||Type:||Article||Affiliation :||Agricultural Research Institute of Cyprus
University of Arizona
Cyprus University of Technology
Aristotle University of Thessaloniki
|Appears in Collections:||Άρθρα/Articles|
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