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Title: Nitric oxide regulates proline and polyamine biosynthesis in medicatgo truncatula plants
Authors: Filippou, Panagiota S. 
Fotopoulos, Vasileios 
Antoniou, Chrystalla 
Category: Biological Sciences
Field: Natural Sciences
Issue Date: Jul-2011
Source: 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants, July 5-8 2011, Budapest Hungary
Conference: International Conference on Reactive Oxygen and Nitrogen Species in Plants 
Abstract: Nitric oxide (NO) is a bioactive molecule involved in numerous biological events that has been reported to act as a signalling molecule regulating a variety of key processes in plants. The present study attempts to examine the effect of application of low (100~) and high (2.5mM) concentrations of sodium nitroprusside (SNP), a NO donor, on the biosynthesis of proline and polyamines, which are known to confer protective characteristics to plants under stress conditions. Analyses were carried out in two developmental stages of Medicago plants (mature and senescing). Spectrophotometric and chromatographic approaches revealed that higher concentrations of SNP induced significant increases in proline and putrescine content following SNP application in both mature and senescing leaves (further induced in older tissues), although spermidine and spermine levels remained unaltered. Increases in proline and putrescine content were supported by increased activities of biosynthetic enzymes P5CS and ADC respectively, although ODC activity displayed similar patterns to control samples. Quantitative real-time RTPCR data examining proline and polyamine biosynthetic gene (P5CS, ADC, ODC, SPMS, SPDS, SAMDC) expression levels suggest that NO plays a key role in the regulation of the relevant transcriptional pathways. Overall, strong evidence suggests that nitric oxide regulates key protective metabolite levels in plants via enzymatic and transcriptional modulation of proline and polyamine biosynthesis pathways, thus potentially improving the plant's capacity to cope with free oxygen and nitrogen radicals.
Type: Conference Papers
Appears in Collections:Δημοσιεύσεις σε συνέδρια /Conference papers - poster -presentation

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