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Title: A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes
Authors: Tzamaloukas, Ouranios 
Goulas, Vlasios 
Angastinioti Moditi, Chrystalleni 
Andreou, Anisimova 
Major Field of Science: Agricultural Sciences
Field Category: Animal and Dairy Science
Keywords: Additives;Antibiotics;Feed premixes;Medicated feed;Sulpha
Issue Date: 11-Jun-2014
Source: Journal of Animal and Feed Sciences, 2014, vol. 23, no. 2, pp. 185-189
Volume: 23
Issue: 2
Start page: 185
End page: 189
Journal: Journal of Animal and Feed Sciences 
Abstract: A simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed premixes. Ultrasound-assisted extraction was used for the recovery of the studied antibiotics without the need for a further clean-up step. The sulphonamides and trimethoprim were separated on a Symmetry C18 (5 μm, 4.6 × 250 mm) column using 10-min isocratic elution and UV detection at 254 nm. The results showed that the method is robust with adequate recovery (minimum 95.19%), satisfactory repeatability (1.39%-2.69%) and intermediate precision (1.36%-4.16%), as shown by the validation assessment herein. The advantages of the proposed method are the speed, the ease of the clean-up protocol, and the low cost of the needed instrumentation, rendering it highly applicable to the feed industry.
ISSN: 1230-1388
DOI: 10.22358/jafs/65708/2014
Collaboration : Cyprus University of Technology
VitaTrace Nutrition Ltd
Rights: © Bentus
Type: Article
Appears in Collections:Άρθρα/Articles

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