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Title: Regulation of ascorbate-glutathione cycle by exogenous nitric oxide and hydrogen peroxide in soybean roots under arsenate stress
Authors: Singh, Samiksha 
Husain, Tajammul 
Kushwaha, Bishwajit Kumar 
Suhel, Mohammad 
Fatima, Abreeq 
Mishra, Vipul 
Singh, Sani Kumar 
Bhatt, Javaid Akhtar 
Rai, Meena 
Prasad, Sheo Mohan 
Dubey, Nawal Kishore 
Chauhan, Devendra Kumar 
Tripathi, Durgesh Kumar 
Fotopoulos, Vasileios 
Singh, Vijay Pratap 
Major Field of Science: Agricultural Sciences
Field Category: Agriculture Forestry and Fisheries
Keywords: Ascorbate-glutathione cycle;Cell death;Oxidative stress;Root growth;Sequestration;Vacuolar
Issue Date: 5-May-2021
Source: Journal of Hazardous Materials, 2021, vol. 409, aticl. no. 123686
Volume: 409
Journal: Journal of Hazardous Materials 
Abstract: The role of nitric oxide (NO) and hydrogen peroxide (H2O2) is well known for regulating plant abiotic stress responses. However, underlying mechanisms are still poorly understood. Therefore, the present study investigated the involvement of NO and H2O2 signalling in the regulation of arsenate toxicity (AsV) in soybean roots employing a pharmacological approach. Results show that AsV toxicity declined root length and biomass due to greater As accumulation in the cell wall and cellular organelles. Arsenate induced cell death due to enhanced levels of reactive oxygen species, lipid and protein oxidation and down-regulation in ascorbate-glutathione cycle and redox states of ascorbate and glutathione. These results correlate with lower endogenous level of NO. Interestingly, addition of L-NAME increased AsV toxicity. However, addition of SNP reverses effect of L-NAME, suggesting that endogenous NO has a role in mitigating AsV toxicity. Exogenous H2O2 also demonstrated capability of alleviating AsV stress, while NAC reversed the protective effect of H2O2. Furthermore, DPI application further increased AsV toxicity, suggesting that endogenous H2O2 is also implicated in mitigating AsV stress. SNP was not able to mitigate AsV toxicity in the presence of DPI, suggesting that H2O2 might have acted downstream of NO in accomplishing amelioration of AsV toxicity.
ISSN: 0304-3894
DOI: 10.1016/j.jhazmat.2020.123686
Rights: © Elsevier
Attribution-NonCommercial-NoDerivatives 4.0 International
Type: Article
Affiliation : University of Allahabad 
Nanjing Agricultural University 
Banaras Hindu University 
Amity University Uttar Pradesh 
Cyprus University of Technology 
Appears in Collections:Άρθρα/Articles

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