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Title: Detection of Maillard reaction products by a coupled HPLC-Fraction collector technique and FTIR characterization of Cu(II)-complexation with the isolated species
Authors: Ioannou, Aristos 
Daskalakis, Evangelos 
Varotsis, Constantinos 
Keywords: Maillard reaction;Asparagine;HPLC;Fraction collector;UV-vis;ATR-FTIR
Category: Environmental Engineering;Other Engineering and Technologies
Field: Natural Sciences
Issue Date: 5-Apr-2017
Source: Journal of Molecular Structure, 2017
Journal: Journal of Molecular Structure 
Abstract: © 2017 Elsevier B.V. The isolation of reaction products of asparagine with reducing sugars at alkaline pH and high temperature has been probed by a combination of high performance liquid chromatography (HPLC) coupled with a Fraction Collector. The UV–vis and FTIR spectra of the isolated Maillard reaction products showed structure-sensitive changes as depicted by deamination events and formation of asparagine-saccharide conjugates. The initial reaction species of the Asn-Gluc reaction were also characterized by Density Functional Theory (DFT) methods. Evidence for Cu (II) metal ion complexation with the Maillard reaction products is supported by UV–vis and FTIR spectroscopy.
ISSN: 00222860
DOI: 10.1016/j.molstruc.2017.04.011
Type: Article
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