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Title: Molecular and chromosomal organization of two repetitive DNA sequences with intercalary locations in sugar beet and other Beta species
Authors: Schmidt, T. 
Kubis, S. 
Katsiotis, Andreas 
Jung, C. 
Heslop-Harrison, J. S. 
Major Field of Science: Agricultural Sciences
Field Category: AGRICULTURAL SCIENCES;Agricultural Biotechnology;Other Agricultural Sciences
Keywords: Beta vulgaris;Dispersed repeats;Fluorescent in situ hybridization;Repetitive DNA;Satellite DNA
Issue Date: Oct-1998
Source: Theoretical and Applied Genetics,1998, vol. 97, no. 5-6, pp. 696-704
Volume: 97
Issue: 5-6
Start page: 696
End page: 704
Journal: Theoretical and Applied Genetics 
Abstract: In a search for repetitive DNA sequences in the sugar beet genome, two sequences with repeat unit lengths of 143 and 434 bp were isolated and characterized. The pSV family showed an unusual conservation of restriction sites reflecting homogenization of the analyzed repeats. Members of the family are organized as tandem repeats as revealed by PCR and sequencing of dimeric units. The pSV satellite occurs in large intercalary arrays which are present on all chromosome arms of sugar beet. The pSV sequence family is present in different abundance in the sections Beta, Corollinae and Nanae but is not detectable by Southern hybridization in the section Procumbentes. The pDRV family is characterized by an interspersed genomic organization. The sequence is detectable in all sections of the genus and is amplified in species of the section Beta but was also detected, although at lower abundance, in the remaining three sections. Fluorescent in situ hybridization has shown that the pDRV sequence family is dispersed over all chromosomes of the sugar beet complement with some regions of clustering and centromeric depletion.
ISSN: 0040-5752
DOI: 10.1007/s001220050945
Rights: © Springer
Type: Article
Affiliation : University of Kiel 
John Innes Centre 
Agricultural University of Athens 
Appears in Collections:Άρθρα/Articles

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