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Title: Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures
Authors: Prokopi, Marianna 
Pula, Giordano 
Mayr, Ursula 
Devue, Cécile 
Gallagher, Joy 
Xiao, Qingzhong 
Boulanger, Chantal M 
Westwood, Nigel 
Urbich, Carmen 
Willeit, Johann 
Steiner, Marianne 
Breuss, Johannes 
Xu, Qingbo 
Kiechl, Stefan 
Mayr, Manuel 
Major Field of Science: Engineering and Technology
Field Category: Mechanical Engineering
Keywords: Circulating EPCs;Stem Cells;Endothelial Cells
Issue Date: 16-Jul-2009
Source: Blood, 2009, vol. 114, no. 3, pp. 723-732
Volume: 114
Issue: 3
Start page: 723
End page: 732
Journal: Blood 
Abstract: The concept of endothelial progenitor cells (EPCs) has attracted considerable interest in cardiovascular research, but despite a decade of research there are still no specific markers for EPCs and results from clinical trials remain controversial. Using liquid chromatography-tandem mass spectrometry, we analyzed the protein composition of microparticles (MPs) originating from the cell surface of EPC cultures. Our data revealed that the conventional methods for isolating mononuclear cells lead to a contamination with platelet proteins. Notably, platelets readily disintegrate into platelet MPs. These platelet MPs are taken up by the mononuclear cell population, which acquires "endothelial" characteristics (CD31, von Willebrand factor [VWF], lectin-binding), and angiogenic properties. In a large population-based study (n = 526), platelets emerged as a positive predictor for the number of colony-forming units and early outgrowth EPCs. Our study provides the first evidence that the cell type consistent with current definitions of an EPC phenotype may arise from an uptake of platelet MPs by mononuclear cells resulting in a gross misinterpretation of their cellular progeny. These findings demonstrate the advantage of using an unbiased proteomic approach to assess cellular phenotypes and advise caution in attributing the benefits in clinical trials using unselected bone marrow mononuclear cells (BMCs) to stem cell-mediated repair.
ISSN: 1528-0020
DOI: 10.1182/blood-2009-02-205930
Rights: © The American Society of Hematology
Type: Article
Affiliation : King's College London 
Université Paris-Descartes 
King's College Hospital 
University of Frankfurt 
Innsbruck Medical University 
Medical University of Vienna 
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