Please use this identifier to cite or link to this item: https://ktisis.cut.ac.cy/handle/10488/1115
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dc.contributor.authorDaras, Gerasimosen
dc.contributor.authorRigas, Stamatisen
dc.contributor.authorPenning, Bryanen
dc.contributor.authorMcCann, Maureen C.en
dc.contributor.authorCarpita, Nicholas C.en
dc.contributor.authorFasseas, Constantinosen
dc.contributor.authorHatzopoulos, Polydefkisen
dc.contributor.authorMilioni, Dimitra L.-
dc.contributor.otherΜηλιώνη, Δήμητρα-
dc.date.accessioned2009-12-21T11:20:11Zen
dc.date.accessioned2013-05-16T13:10:59Z-
dc.date.accessioned2015-12-02T09:39:07Z-
dc.date.available2009-12-21T11:20:11Zen
dc.date.available2013-05-16T13:10:59Z-
dc.date.available2015-12-02T09:39:07Z-
dc.date.issued2009en
dc.identifier.citationNew Phytologist. Volume 184 Issue 1, Pages 114 - 126en
dc.identifier.urihttp://ktisis.cut.ac.cy/handle/10488/1115en
dc.description.abstractGenetic functional analyses of mutants in plant genes encoding cellulose synthases (CesAs) have suggested that cellulose deposition requires the activity of multiple CesA proteins. Here, a genetic screen has led to the identification of thanatos (than), a semi-dominant mutant of Arabidopsis thaliana with impaired growth of seedlings. Homozygous seedlings of than germinate and grow but do not survive. In contrast to other CesA mutants, heterozygous plants are dwarfed and display a radially swollen root phenotype. Cellulose content is reduced by approximately one-fifth in heterozygous and by two-fifths in homozygous plants, showing gene-dosage dependence. Map-based cloning revealed an amino acid substitution (P578S) in the catalytic domain of the AtCesA3 gene, indicating a critical role for this residue in the structure and function of the cellulose synthase complex. Ab initio analysis of the AtCesA3 subdomain flanking the conserved proline residue predicted that the amino acid substitution to serine alters protein secondary structure in the catalytic domain. Gene dosage-dependent expression of the AtCesA3 mutant gene in wild-type A. thaliana plants resulted in a than dominant-negative phenotype. We propose that the incorporation of a mis-folded CesA3 subunit into the cellulose synthase complex may stall or prevent the formation of functional rosette complexes.en
dc.formatpdfen
dc.language.isoenen
dc.publisherNew Phytologist Trusten
dc.rights© 2010 New Phytologist Trusten
dc.subjectArabidopsis thalianaen
dc.subjectCellulose biosynthesisen
dc.subjectCellulose synthasesen
dc.subjectPlant growthen
dc.subjectPrimary cell wallen
dc.subjectSemi-dominant mutationen
dc.titleThe thanatos mutation in Arabidopsis thaliana cellulose synthase 3 (AtCesA3) has a dominant-negative effect on cellulose synthesis and plant growthen
dc.typeArticleen
dc.collaborationAgricultural University of Athens-
dc.countryGreece-
dc.identifier.doi10.1111/j.1469-8137.2009.02960.xen
dc.dept.handle123456789/54en
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.languageiso639-1other-
crisitem.author.deptDepartment of Communication and Internet Studies-
crisitem.author.facultyFaculty of Communication and Media Studies-
crisitem.author.orcid0000-0002-2342-4952-
crisitem.author.parentorgFaculty of Communication and Media Studies-
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