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Title: Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents
Authors: Koutinas, Michalis 
Yiangou, Chrystalleni 
Osório, Natália M. 
Ioannou, Katerina 
Canet, Albert 
Valero, Francisco 
Ferreira-Dias, Suzana 
Major Field of Science: Engineering and Technology
Field Category: Industrial Biotechnology
Keywords: Esterification;Ethyl lactate;Lipase;Novozym 435;Operational stability
Issue Date: Jan-2018
Source: Bioresource Technology, 2018, vol. 247, pp. 496-503
Volume: 247
Start page: 496
End page: 503
Journal: Bioresource Technology 
Abstract: This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0.01–0.1 M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent’s Log P increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.
ISSN: 0960-8524
DOI: 10.1016/j.biortech.2017.09.130
Rights: © Elsevier
Type: Article
Affiliation : Cyprus University of Technology 
Escola Superior de Tecnologia do Barreiro 
Universitat Autònoma de Barcelona 
Universidade de Lisboa 
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