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|Title:||Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents||Authors:||Koutinas, Michalis
Osório, Natália M.
|Major Field of Science:||Engineering and Technology||Field Category:||Industrial Biotechnology||Keywords:||Esterification;Ethyl lactate;Lipase;Novozym 435;Operational stability||Issue Date:||Jan-2018||Source:||Bioresource Technology, 2018, vol. 247, pp. 496-503||Volume:||247||Start page:||496||End page:||503||Journal:||Bioresource Technology||Abstract:||This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0.01–0.1 M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent’s Log P increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.||ISSN:||0960-8524||DOI:||10.1016/j.biortech.2017.09.130||Rights:||© Elsevier||Type:||Article||Affiliation :||Cyprus University of Technology
Escola Superior de Tecnologia do Barreiro
Universitat Autònoma de Barcelona
Universidade de Lisboa
|Appears in Collections:||Άρθρα/Articles|
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