Please use this identifier to cite or link to this item: https://ktisis.cut.ac.cy/handle/10488/10978
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dc.contributor.authorMarti, Angel Fernandez Ι.-
dc.contributor.authorSaski, Christopher A.-
dc.contributor.authorManganaris, George A.-
dc.contributor.authorGasic, Ksenija-
dc.contributor.authorCrisosto, Carlos H.-
dc.date.accessioned2018-04-30T07:40:08Z-
dc.date.available2018-04-30T07:40:08Z-
dc.date.issued2018-01-19-
dc.identifier.citationFrontiers in Plant Science, 2018, vol. 9en_US
dc.identifier.issn1664-462X-
dc.description.abstractIt has recently been described that the Japanese plum “Santa Rosa” bud sport series contains variations in ripening pattern: climacteric, suppressed-climacteric and non-climacteric types. This provides an interesting model to study the role of ethylene and other key mechanisms governing fruit ripening, softening and senescence. The aim of the current study was to investigate such differences at the genomic level, using this series of plum bud sports, with special reference to genes involved in ethylene biosynthesis, signal transduction, and sugar metabolism. Genomic DNA, isolated from leaf samples of six Japanese plumcultivars (“Santa Rosa”, “July Santa Rosa”, “Late Santa Rosa”, “Sweet Miriam”, “Roysum”, and “Casselman”), was used to construct paired-end standard Illumina libraries. Sequences were aligned to the Prunus persica genome, and genomic variations (SNPs, INDELS, andCNV’s) were investigated. Results determined12 potential candidate genes with significant copy number variation (CNV), being associated with ethylene perception andsignal transduction components. Additionally, the Maximum Likelihood (ML) phylogenetic tree showed two sorbitol dehydrogenase genes grouping into a distinct clade, indicating that this natural group is well-defined and presents high sequence identity among its members. In contrast, the ethylene group, which includes ACO1, ACS1, ACS4, ACS5, CTR1, ERF1, ERF3, and ethylene-receptor genes, was widely distributed and clustered into 10 different groups. Thus, ACS, ERF, and sorbitol dehydrogenase proteins potentially share a common ancestor for different plant genomes, while the expansion rate may be related to ancestral expansion rather than species-specific events. Based on the distribution of the clades, we suggest that gene function diversification for the ripening pathway occurred prior to family extension. We herein report all the frameshift mutations in genes involved in sugar transport and ethylene biosynthesis detected as well as the gene CNV implicated in ripening differences.en_US
dc.formatpdfen_US
dc.language.isoenen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Plant Scienceen_US
dc.rights© Fernandez i Marti, Saski, Manganaris, Gasic and Crisostoen_US
dc.subjectClimactericen_US
dc.subjectCopy number variationen_US
dc.subjectMolecular evolutionen_US
dc.subjectNon-climactericen_US
dc.subjectPE Illumina librariesen_US
dc.subjectRipeningen_US
dc.subjectSNPs/INDELSen_US
dc.subjectSuppressed-climactericen_US
dc.titleGenomic sequencing of Japanese plum (Prunus salicina Lindl.) mutants provides a new model for rosaceae fruit ripening studiesen_US
dc.typeArticleen_US
dc.doihttps://doi.org/10.3389/fpls.2018.00021en_US
dc.collaborationUniversity of Californiaen_US
dc.collaborationBiosystems Research Complexen_US
dc.collaborationCyprus University of Technologyen_US
dc.collaborationClemson Universityen_US
dc.subject.categoryAgricultural Biotechnologyen_US
dc.journalsOpen Accessen_US
dc.countryUnited Statesen_US
dc.countryCyprusen_US
dc.subject.fieldAgricultural Sciencesen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.3389/fpls.2018.00021en_US
dc.relation.volume9en_US
cut.common.academicyear2017-2018en_US
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.openairetypearticle-
item.languageiso639-1en-
item.cerifentitytypePublications-
crisitem.journal.journalissn1664-462X-
crisitem.journal.publisherFrontiers-
crisitem.author.deptDepartment of Agricultural Sciences, Biotechnology and Food Science-
crisitem.author.facultyFaculty of Geotechnical Sciences and Environmental Management-
crisitem.author.orcid0000-0002-5849-6104-
crisitem.author.parentorgFaculty of Geotechnical Sciences and Environmental Management-
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