Please use this identifier to cite or link to this item:
|Title:||Bioconversion of alkaloids to high-value chemicals: Comparative analysis of newly isolated lupanine degrading strains||Authors:||Parmaki, Stella
Barbeitos, Catarina B.M
Ferreira, Frederico C.
Afonso, Carlos A.M.
|Keywords:||Bioconversion;Biodegradation;Lupanine;Quinolizidine alkaloids;Toxicity assessment||Category:||Chemical Sciences||Field:||Natural Sciences||Issue Date:||Feb-2018||Publisher:||Elsevier Ltd.||Source:||Chemosphere, 2018, Volume 193, Pages 50-59||DOI:||https://doi.org/10.1016/j.chemosphere.2017.10.165||Journal:||Chemosphere||Abstract:||This work explores the potential for development of a lupanine valorization process evaluating different isolated microorganisms for their capacity to metabolize the alkaloid. Ecotoxicological assessment demonstrated that lupanine is toxic for Vibrio fischeri and Daphnia magna exhibiting EC50 values of 89 mg L−1 and 47 mg L−1 respectively, while acting both as growth inhibitor for a monocotyledonous and as promoter for a dicotyledonous plant. Among the eight aerobic and anaerobic strains isolated and identified Rhodococcus rhodochrous LPK211 achieved 81% removal for 1.5 g L−1 lupanine, while no end-products were detected by NMR constituting a promising microorganism for lupanine biodegradation. Moreover, Rhodococcus ruber LPK111 and Rhodococcus sp. LPK311 exhibited 66% and 71% of removal respectively, including potential formation of lupanine N-oxide. Pseudomonas putida LPK411 reached 80% of lupanine removal and generated three fermentation products potentially comprising 17-oxolupanine and lupanine derivatives with open ring structures enabling the development of alkaloid valorization processes.||URI:||http://ktisis.cut.ac.cy/handle/10488/10971||ISSN:||0045-6535||DOI:||10.1016/j.chemosphere.2017.10.165||Rights:||© 2017 Elsevier Ltd||Type:||Article|
|Appears in Collections:||Άρθρα/Articles|
Show full item record
WEB OF SCIENCETM
checked on Jun 15, 2019
Page view(s) 5068
checked on Jun 25, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.