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Title: Bioconversion of alkaloids to high-value chemicals: Comparative analysis of newly isolated lupanine degrading strains
Authors: Parmaki, Stella 
Vyrides, Ioannis 
Vasquez Christodoulou, Marlen 
Hartman, Viki 
Zacharia, Irene 
Hadjiadamou, Ioanna 
Barbeitos, Catarina B.M 
Ferreira, Frederico C. 
Afonso, Carlos A.M. 
Drouza, Chryssoula 
Koutinas, Michalis 
Keywords: Bioconversion;Biodegradation;Lupanine;Quinolizidine alkaloids;Toxicity assessment
Category: Chemical Sciences
Field: Natural Sciences
Issue Date: Feb-2018
Publisher: Elsevier Ltd.
Source: Chemosphere, 2018, Volume 193, Pages 50-59
Journal: Chemosphere 
Abstract: This work explores the potential for development of a lupanine valorization process evaluating different isolated microorganisms for their capacity to metabolize the alkaloid. Ecotoxicological assessment demonstrated that lupanine is toxic for Vibrio fischeri and Daphnia magna exhibiting EC50 values of 89 mg L−1 and 47 mg L−1 respectively, while acting both as growth inhibitor for a monocotyledonous and as promoter for a dicotyledonous plant. Among the eight aerobic and anaerobic strains isolated and identified Rhodococcus rhodochrous LPK211 achieved 81% removal for 1.5 g L−1 lupanine, while no end-products were detected by NMR constituting a promising microorganism for lupanine biodegradation. Moreover, Rhodococcus ruber LPK111 and Rhodococcus sp. LPK311 exhibited 66% and 71% of removal respectively, including potential formation of lupanine N-oxide. Pseudomonas putida LPK411 reached 80% of lupanine removal and generated three fermentation products potentially comprising 17-oxolupanine and lupanine derivatives with open ring structures enabling the development of alkaloid valorization processes.
ISSN: 0045-6535
DOI: 10.1016/j.chemosphere.2017.10.165
Rights: © 2017 Elsevier Ltd
Type: Article
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