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Title: Detection of Maillard reaction products by a coupled HPLC-Fraction collector technique and FTIR characterization of Cu(II)-complexation with the isolated species
Authors: Ioannou, Aristos 
Daskalakis, Evangelos 
Varotsis, Constantinos 
Keywords: Asparagine;ATR-FTIR;Fraction collector;HPLC;Maillard reaction;UV–vis
Category: Earth and Related Environmental Sciences
Field: Natural Sciences
Issue Date: 5-Aug-2017
Publisher: Elsevier B.V.
Source: Journal of Molecular Structure, 2017, Volume 1141, Pages 634-642
Abstract: The isolation of reaction products of asparagine with reducing sugars at alkaline pH and high temperature has been probed by a combination of high performance liquid chromatography (HPLC) coupled with a Fraction Collector. The UV–vis and FTIR spectra of the isolated Maillard reaction products showed structure-sensitive changes as depicted by deamination events and formation of asparagine-saccharide conjugates. The initial reaction species of the Asn-Gluc reaction were also characterized by Density Functional Theory (DFT) methods. Evidence for Cu (II) metal ion complexation with the Maillard reaction products is supported by UV–vis and FTIR spectroscopy.
ISSN: 00222860
DOI: 10.1016/j.molstruc.2017.04.011
Rights: © 2017 Elsevier B.V.
Type: Article
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