Please use this identifier to cite or link to this item: https://ktisis.cut.ac.cy/handle/10488/10069
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dc.contributor.authorAllsop, Thomas P.-
dc.contributor.authorMou, Chengbo-
dc.contributor.authorNeal, Ron M.-
dc.contributor.authorMariani, Stefano-
dc.contributor.authorNagel, David Andrew-
dc.contributor.authorTombelli, Sara-
dc.contributor.authorPoole, Andrew J.-
dc.contributor.authorKalli, Kyriacos-
dc.contributor.authorHine, Anna V.-
dc.contributor.authorWebb, David J.-
dc.contributor.authorCulverhouse, Phil-
dc.contributor.authorMinunni, Maria-
dc.contributor.authorBennion, I.-
dc.contributor.otherΚαλλή, Κυριάκος-
dc.date.accessioned2017-04-25T11:44:47Z-
dc.date.available2017-04-25T11:44:47Z-
dc.date.issued2017-01-09-
dc.identifier.citationOptics Express, 2017, vol. 25, no. 1, pp. 39-58en_US
dc.identifier.issn1094-4087-
dc.description.abstractHere we present a new generic opto-bio-sensing platform combining immobilised aptamers on an infrared plasmonic sensing device generated by nano-structured thin film that demonstrates amongst the highest index spectral sensitivities of any optical fibre sensor yielding on average 3.4 × 104 nm/RIU in the aqueous index regime (with a figure of merit of 330) This offers a single stage, solution phase, atto-molar detection capability, whilst delivering real-time data for kinetic studies in water-based chemistry. The sensing platform is based upon optical fibre and has the potential to be multiplexed and used in remote sensing applications. As an example of the highly versatile capabilities of aptamer based detection using our platform, purified thrombin is detected down to 50 attomolar concentration using a volume of 1mm3 of solution without the use of any form of enhancement technique. Moreover, the device can detect nanomolar levels of thrombin in a flow cell, in the presence of 4.5% w/v albumin solution. These results are important, covering all concentrations in the human thrombin generation curve, including the problematic initial phase. Finally, selectivity is confirmed using complementary and non-complementary DNA sequences that yield performances similar to those obtained with thrombin.en_US
dc.formatpdfen_US
dc.language.isoenen_US
dc.publisherOSA - The Optical Societyen_US
dc.relation.ispartofOptics Expressen_US
dc.rights©2016 Optical Society of America.en_US
dc.subjectOpto-bio-sensing platformen_US
dc.subjectRemote sensingen_US
dc.subjectDNA sequencesen_US
dc.titleReal-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmonsen_US
dc.typeArticleen_US
dc.doi10.1364/OE.25.000039en_US
dc.collaborationAston Universityen_US
dc.collaborationShanghai Universityen_US
dc.collaborationUniversity of Plymouthen_US
dc.collaborationUniversita degli Studi di Firenzeen_US
dc.collaborationIstituto Di Fisica Applicata Nello Carraraen_US
dc.collaborationCyprus University of Technologyen_US
dc.subject.categoryElectrical Engineering - Electronic Engineering - Information Engineeringen_US
dc.journalsOpen Accessen_US
dc.countryUnited Kingdomen_US
dc.countryChinaen_US
dc.countryItalyen_US
dc.countryCyprusen_US
dc.subject.fieldEngineering and Technologyen_US
dc.publicationPeer Revieweden_US
dc.identifier.doi10.1364/OE.25.000039en_US
cut.common.academicyear2016-2017en_US
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.languageiso639-1other-
crisitem.journal.journalissn1094-4087-
crisitem.journal.publisherOSA-
crisitem.author.deptDepartment of Electrical Engineering, Computer Engineering and Informatics-
crisitem.author.facultyFaculty of Engineering and Technology-
crisitem.author.parentorgFaculty of Engineering and Technology-
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