Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/9851
Title: Detection of Mycobacterium avium subsp. paratuberculosis in bulk tank milk by combined phage-PCR assay: Evidence that plaque number is a good predictor of MAP
Authors: Botsaris, George 
Liapi, Maria 
Kakogiannis, Charalambos 
Dodd, Christine E R 
Rees, Catherine E.D. 
Keywords: Detection
Milk
Paratuberculosis
Phage-PCR assay
Issue Date: 3-Jun-2013
Publisher: Elsevier
Source: International Journal of Food Microbiology, 2013, Volume 164, Issue 1, Pages 76-80
Abstract: Conventional culture and a rapid phage-PCR method were used to detect Mycobacterium avium subsp. paratuberculosis (MAP) in bulk tank milk (BTM) samples. Only two of 225 samples (0.9%) were found to contain MAP by culture whereas 50 (22%) MAP-positive samples were identified using the phage-PCR assay, including both samples that were MAP-culture positive. Results using the phage-based method for independently tested duplicate samples indicated that the assay is very reproducible (r2=0.897), especially when low levels of mycobacteria are present. A relationship was established between plaque number and the presence of MAP in a sample. A cut-off value was determined allowing identification of MAP-positive samples based on plaque number alone (90% sensitivity, 99% specificity; area under the curve=0.976). These results indicate that the assay is a robust method for screening BTM, providing results within 24h.
URI: http://ktisis.cut.ac.cy/handle/10488/9851
ISSN: 01681605
Rights: © 2013 Elsevier B.V.
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