Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/9355
Title: Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants
Authors: Ziogas, Vasileios 
Tanou, Georgia 
Belghazi, Maya 
Filippou, Panagiota S. 
Fotopoulos, Vasileios 
Grigorios, Diamantidis 
Molassiotis, Athanassios N. 
Keywords: Citrus
Drought stress
Plant proteomics
Priming
Sodium hydrosulfide
Sodium nitroprusside
Issue Date: 24-Sep-2015
Publisher: Springer Netherlands
Source: Plant Molecular Biology, 2015, Volume 89, Issue 4-5, Pages 433-450
Abstract: Emerging evidence suggests that the gaseous molecules hydrogen sulfide (H2S) and nitric oxide (NO) enhances plant acclimation to stress; however, the underlying mechanism remains unclear. In this work, we explored if pretreatment of citrus roots with NaHS (a H2S donor) or sodium nitroprusside (SNP, a NO donor) for 2 days (d) could elicit long-lasting priming effects to subsequent exposure to PEG-associated drought stress for 21 d following a 5 d acclimation period. Detailed physiological study documented that both pretreatments primed plants against drought stress. Analysis of the level of nitrite, NOx, S-nitrosoglutahione reductase, Tyr-nitration and S-nitrosylation along with the expression of genes involved in NO-generation suggested that the nitrosative status of leaves and roots was altered by NaHS and SNP. Using a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chemical treatments, including well known and novel S-nitrosylated targets. Mass spectrometry analysis also enabled the identification of 42 differentially expressed proteins in PEG alone-treated plants. Several PEG-responsive proteins were down-regulated, especially photosynthetic proteins. Finally, the identification of specific proteins that were regulated by NaHS and SNP under PEG conditions provides novel insight into long-term drought priming in plants and in a fruit crop such as citrus in particular.
URI: http://ktisis.cut.ac.cy/handle/10488/9355
ISSN: 01674412
Rights: © 2015, Springer Science+Business Media Dordrecht.
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