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Title: Bioanalytical LC-MS Method for the Quantification of Plasma Androgens and Androgen Glucuronides in Breast Cancer
Authors: Kalogera, Eleni 
Pistos, Constantinos M. 
Provatopoulou, Xeni 
Christophi, Costas A. 
Zografos, George Constantine 
Stefanidou, Maria E. 
Spiliopoulou, Chara A. 
Athanaselis, Sotiris A. 
Gounaris, Antonia D. 
Keywords: Breast cancer;Plasma androgens;Androgen glucuronides
Category: Health Sciences
Field: Medical and Health Sciences
Issue Date: 1-Apr-2016
Publisher: Oxford University Press
Source: Journal of Chromatographic Science, 2016, Volume 54, Issue 4, Pages 583-592
metadata.dc.doi: 10.1093/chromsci/bmv190
Abstract: The physiological and pathological development of the breast is strongly affected by the hormonal milieu consisting of steroid hormones. Mass spectrometry (MS) technologies of high sensitivity and specificity enable the quantification of androgens and consequently the characterization of the hormonal status. The aim of this study is the assessment of plasma androgens and androgen glucuronides, in the par excellence hormone-sensitive tissue of the breast, through the application of liquid chromatography-mass spectrometry (LC-MS). A simple and efficient fit-for-purpose method for the simultaneous identification and quantification of dehydroepiandrosterone sulfate (DHEAS), androstenedione (A4), androsterone glucuronide (ADTG) and androstane-3α, 17β-diol-17-glucuronide (3α-diol-17G) in human plasma was developed and validated. The presented method permits omission of derivatization, requires a single solid-phase extraction procedure and the chromatographic separation can be achieved on a single C18 analytical column, for all four analytes. The validated method was successfully applied for the analysis of 191 human plasma samples from postmenopausal women with benign breast disease (BBD), lobular neoplasia (LN), ductal carcinoma in situ and invasive ductal carcinoma (IDC). DHEAS plasma levels exhibited significant differences between LN, IDC and BBD patients (P < 0.05). Additionally, ADTG levels were significantly higher in patients with LN compared with those with BBD (P < 0.05).
ISSN: 00219665
Rights: © 2016 The Author
Type: Article
Appears in Collections:Άρθρα/Articles

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