Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/918
Title: Altered ascorbate redox state in tobacco plants results in delayed dark induced senescence in detached leaves
Authors: Fotopoulos, Vasileios 
Kanellis, Angelos K. 
Issue Date: 2009
Source: Plant Abiotic Stress from signaling to development, 2nd meeting of the INPAS 14-17 May 2009 Tartu Estonia
Abstract: Ascorbate oxidase (AO) is an apoplastic enzyme that uses oxygen to catalyze the oxidation of ascorbate (AA) to dehydroascorbate (DHA) via the unstable radical monodehydroascorbate (MDHA). Here, we report that transgenic tobacco plants (Nicotiana tabacum L. cv. Xanthi) with an in vivo lowered apoplast AA redox state through increased AO expression demonstrate signs of delayed dark-induced senescence compared with wild-type plants, as shown by chlorophyll loss and lipid peroxidation assays. In situ localization of H2O2 suggests that although transgenic plants have higher constitutive levels of H2O2 under normal growth conditions, imposed dark-induced senescence results in smaller induction levels of H2O2, an observation which correlates with increased antioxidant enzyme activities and an induction in the expression of AA recycling genes compared with that in wild-type plants. Our current findings, combined with previous studies which showed the contribution of AO in the regulation of AA redox state, suggest that the reduction in AA redox state in the leaf apoplast of these transgenic plants results in an increase in the endogenous levels of H2O2, which provides a form of ‘acquired tolerance’ to oxidative stress imposed by dark-induced senescence.
Appears in Collections:Περιλήψεις/Abstracts

Files in This Item:
File Description SizeFormat 
INPAS Estonia Meeting Abstract 1.pdf224.95 kBAdobe PDFView/Open
Show full item record

Page view(s) 20

4
checked on Jan 6, 2017

Download(s) 20

1
checked on Jan 6, 2017

Google ScholarTM

Check


This item is licensed under a Creative Commons License Creative Commons