Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/9068
Title: Detection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by culture
Authors: Botsaris, George 
Swift, Benjamin M. C. 
Slana, Iva 
Liapi, Maria 
Christodoulou, Maritsa 
Hatzitofi, Maria 
Christodoulou, Vasiliki 
Rees, Catherine 
Keywords: PCR
Paratuberculosis
Pasteurised milk
Phage
Powdered infant formula
Rapid methods
Issue Date: 4-Jan-2016
Publisher: Elsevier Ltd
Source: International Journal of Food Microbiology, January 04 2016, Volume 216, Pages 91-94
Abstract: Surveys from different parts of the world have reported that viable Mycobacterium avium subsp. paratuberculosis (MAP) can be cultured from approximately 2% of samples of retail pasteurised milk samples. Pasteurised milk is used for the production of powdered infant formula (PIF) and therefore there is a concern that MAP may also be present in these products. Several studies have previously reported the detection of MAP in PIF using PCR-based assays. However, culture-based surveys of PIF have not detected viable MAP. Here we describe a phage amplification assay coupled with PCR (page-PCR) that can rapidly detect viable MAP in PIF. The results of a small survey showed that the phage-PCR assay detected viable MAP in 13% (4/32) of PIF samples. Culture detected viable MAP in 9% (3/32) PIF samples, all of which were also phage-PCR positive. Direct IS. 900 PCR detected MAP DNA in 22% (7/32) of PIF samples. The presence of viable MAP in PIF indicates that MAP either survived PIF manufacturing or that post-production contamination occurred. Irrespective of the route of MAP contamination, the presence of viable MAP in PIF is a potential public health concern.
URI: http://ktisis.cut.ac.cy/handle/10488/9068
ISSN: 01681605
Rights: © 2015 Elsevier B.V.
Appears in Collections:Άρθρα/Articles

Files in This Item:
File Description SizeFormat 
Botsaris.pdfThis is a RoMEO green journal250.6 kBAdobe PDFView/Open
Show full item record

Page view(s) 20

28
Last Week
1
Last month
2
checked on Jul 26, 2017

Download(s) 20

10
checked on Jul 26, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.