Please use this identifier to cite or link to this item:
Title: Selective one-dimensional total correlation spectroscopy nuclear magnetic resonance experiments for a rapid identification of minor components in the lipid fraction of milk and dairy products: toward spin chromatography?
Authors: Papaemmanouil, Christina
Tsiafoulis, Constantinos G.
Alivertis, Dimitrios
Tzamaloukas, Ouranios 
Tzakos, Andreas G.
Gerothanassis, Ioannis P.
Miltiadou, Despoina 
Keywords: Lipid fraction;Totally correlated NMR spectroscopy (TOCSY);Spin chromatography;Dairy products
Category: Animal and Dairy Science
Field: Agricultural Sciences
Issue Date: 10-Jul-2015
Publisher: ACS Publications
Source: Journal of Agricultural and Food Chemistry, 2015, Volume 63, Issue 22, Pages 5381–5387
Abstract: We report a rapid, direct, and unequivocal spin-chromatographic separation and identification of minor components in the lipid fraction of milk and common dairy products with the use of selective one-dimensional (1D) total correlation spectroscopy (TOCSY) nuclear magnetic resonance (NMR) experiments. The method allows for the complete backbone spin-coupling network to be elucidated even in strongly overlapped regions and in the presence of major components from 4 × 102 to 3 × 103 stronger NMR signal intensities. The proposed spin-chromatography method does not require any derivatization steps for the lipid fraction, is selective with excellent resolution, is sensitive with quantitation capability, and compares favorably to two-dimensional (2D) TOCSY and gas chromatography–mass spectrometry (GC–MS) methods of analysis. The results of the present study demonstrated that the 1D TOCSY NMR spin-chromatography method can become a procedure of primary interest in food analysis and generally in complex mixture analysis.
ISSN: 0021-8561
DOI: 10.1021/acs.jafc.5b01335
Rights: © American Chemical Society
Type: Article
Appears in Collections:Άρθρα/Articles

Show full item record


checked on Dec 17, 2018

Citations 10

checked on Feb 16, 2019

Page view(s) 50

Last Week
Last month
checked on Feb 18, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.