Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/7345
Title: Optical detection of intracellular cavitation during selective laser targeting of the retinal pigment epithelium: dependence of cell death mechanism on pulse duration
Authors: Lee, Ho
Alt, Clemens 
Pitsillides, Costas 
Keywords: Cell death;Cattle;Retina--Diseases
Issue Date: 2007
Publisher: Spie
Source: Journal of biomedical optics, 2007, Volume 12, Issue 6
Abstract: Selective laser targeting of the retinal pigment epithelium (RPE) is an attractive method for treating RPE-associated disorders. We are developing a method for optically detecting intracellular microcavitation that can potentially serve as an immediate feedback of the treatment outcome. Thermal denaturation or intracellular cavitation can kill RPE cells during selective targeting. We examined the cell damage mechanism for laser pulse durations from 1 to 40 μs ex vivo. Intracellular cavitation was detected as a transient increase in the backscattered treatment beam. Cavitation and cell death were correlated for individual cells after single-pulse irradiation. The threshold radiant exposures for cell death (ED50,d) and cavitation (ED50,c) increased with pulse duration and were approximately equal for pulses of up to 10 μs. For 20 μs, the ED50,d was about 10% lower than the ED50,c; the difference increased with 40-μs pulses. Cells were killed predominantly by cavitation (up to 10-μs pulses); probability of thermally induced cell death without cavitation gradually increases with pulse duration. Threshold measurements are discussed by modeling the temperature distribution around laser-heated melanosomes and the scattering function from the resulting cavitation. Detection of intracellular cavitation is a highly sensitive method that can potentially provide real-time assessment of RPE damage during selective laser targeting
URI: http://ktisis.cut.ac.cy/handle/10488/7345
ISSN: 1083-3668
DOI: 10.1117/1.2804078
Rights: © 2007 Society of Photo-Optical Instrumentation Engineers
Type: Article
Appears in Collections:Άρθρα/Articles

Show full item record

WEB OF SCIENCETM
Citations 5

24
Last Week
1
Last month
checked on Nov 23, 2017

Page view(s)

16
Last Week
0
Last month
0
checked on Nov 24, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.