Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/7343
Title: Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
Authors: Li, Chunqiang 
Pastila, Riikka K. 
Pitsillides, Costas 
Keywords: Leucocytes;Fluorescence;Amino acids;Cells;Mice;Photons
Issue Date: 2010
Publisher: The Optical Society
Source: Optics express, 2010, Volume 18, Issue 2, Pages 988-999
Abstract: We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact dynamically with the dermal vascular endothelium. Inflammation significantly enhances leukocyte rolling, adhesion, and tissue infiltration. After exiting the vasculature, leukocytes continue to move actively in tissue as observed by time-lapse microscopy, and are distinguishable from resident autofluorescent cells that are not motile. Because the new method alleviates the need to introduce exogenous labels, it is potentially applicable for tracking leukocytes and monitoring inflammatory cellular reactions in humans
URI: http://ktisis.cut.ac.cy/handle/10488/7343
ISSN: 1094-4087
DOI: 10.1364/OE.18.000988
Rights: © 2010 Optical Society of America
Type: Article
Appears in Collections:Άρθρα/Articles

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