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Title: In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response
Authors: Fan, Zhigang 
Spencer, Joel A. 
Pitsillides, Costas 
Keywords: T cells
Animal experimentation
Monoclonal antibodies
Flow cytometry
Issue Date: 2010
Publisher: Nature
Source: Nature medicine, 2010, Volume 16, Issue 6, Pages 718-722
Abstract: Here we present methods to longitudinally track islet allograft–infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (Teff cells) and discrimination between natural and induced regulatory T cells (nTreg and iTreg cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish real-time cell tracking as a powerful means to probe the dynamic cellular interplay mediating immunologic rejection or transplant tolerance
ISSN: 1078-8956
DOI: 10.1038/nm.2155
Rights: © 2010 Nature America, Inc. All rights reserved
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