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Title: NO loading: Efficiency assessment of five commonly used application methods of sodium nitroprusside in Medicago truncatula plants
Authors: Fotopoulos, Vasileios 
Filippou, Panagiota 
Antoniou, Chrystalla 
Keywords: Medicago
Nitric oxide
Issue Date: 2012
Publisher: Elsevier
Source: Plant physiology and biochemistry, 2012, volume 60, pages 115-118
Abstract: Nitric oxide (NO) is a bioactive, diffusible molecule involved in a multitude of physiological and developmental processes in plants, which has been reported to display both antioxidant and pro-oxidant properties in plants. Several reports exist highlighting the protective action of sodium nitroprusside (SNP), an NO donor, which demonstrate its important role as a signal molecule in plants responsible for the expression regulation of antioxidant and other defense enzymes. However, the mode of application of this compound varies greatly between studies. The present study provides a comprehensive efficiency comparison of the most commonly used application methods using 2.5. mM SNP on mature (40 day) Medicago truncatula plants. Measurement of NO content in both leaves and roots suggests that vacuum infiltration is the most efficient method for NO donation in leaf tissue, whereas hydroponic application resulted in highest NO content in roots. NO content correlated with activity levels of nitrate reductase (NR; EC, a key enzyme involved in the generation of NO in plants and which is known to be regulated by NO itself
ISSN: 09819428
DOI: 10.1016/j.plaphy.2012.07.026
Rights: © 2012 Elsevier Masson SAS
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