Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/6466
Title: Mode of action of a non-pathogenic Fusarium oxysporum strain against Verticillium dahliae using Real Time QPCR analysis and biomarker transformation
Authors: Tjamos, Sotirios
Stringlis, Ioannis A.
Pantelides, Iakovos 
Keywords: Phytopathogenic microorganisms--Biological control;Fungi;Fusarium oxysporum;Eggplant;Verticillium dahliae;Verticillium
Issue Date: 2009
Publisher: Elsevier
Source: Biological control, 2009, volume 50, issue 1, pages 30-36
Abstract: Verticillium wilt is a devastating disease of a wide range of herbaceous and woody plant hosts. It is incited by the soilborne fungus Verticillium dahliae. Management strategies are mainly focused on preventive measures. In a previous study, the efficacy of a non-pathogenic Fusarium oxysporum strain, designated as F2, isolated from a suppressive compost amendment, has been reported to reduce Verticillium wilt symptom development in eggplants under greenhouse and field conditions; in addition, antibiosis or parasitism were ruled out by using a dual culture test. In the present study, we investigated the mode of action of F2 against V. dahliae. For this purpose, the F2 and V. dahliae strains were transformed with the EGFP and DsRed2 reporter genes respectively, so as to visualize their presence on the root surface of eggplants. In addition, the ramification of both fungi into the plant vascular system was monitored by Real Time QPCR analysis. It was shown that F2 colonizes the root surface along the intercellular junctions excluding V. dahliae from the same ecological niche. In parallel, QPCR analysis showed that application of F2 reduces the levels of V. dahliae vascular colonization along with the disease severity. In a split root experiment it was demonstrated that F2 does not trigger the defense mechanisms of eggplants against V. dahliae. Therefore, it seems that competition for space or nutrients on the root surface are the main mechanism of action of F2 against V. dahliae
URI: http://ktisis.cut.ac.cy/handle/10488/6466
ISSN: 10499644
DOI: http://dx.doi.org/10.1016/j.biocontrol.2009.01.010
Rights: © 2009 Elsevier Inc. All rights reserved
Type: Article
Appears in Collections:Άρθρα/Articles

Show full item record

SCOPUSTM   
Citations 10

31
checked on Nov 16, 2017

Page view(s)

22
Last Week
1
Last month
2
checked on Nov 22, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.