Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/6464
Title: Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H +-ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum
Authors: Fotopoulos, Vasileios 
Holmes, Robert
Hall, John L. 
Williams, Lorraine E. 
Keywords: Arabidopsis thaliana
Erysiphales
Plant pathology
Plasma membrane H+-ATPase
Cell membrane
Cloning
Gene expression
Membrane proteins
Phylogeny
Enzymology
Issue Date: 2006
Publisher: Elsevier
Source: Mycological research, 2006, volume 110, issue 1, pages 28-37
Abstract: Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H +-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H +-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen
URI: http://ktisis.cut.ac.cy/handle/10488/6464
ISSN: 09537562
DOI: 10.1016/j.mycres.2005.08.011
Rights: © The British Mycological Society
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