Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/4804
Title: Effect of drought and rewatering on the cellular status and antioxidant response of Medicago truncatula plants
Authors: Filippou, Panagiota 
Fotopoulos, Vasileios 
Antoniou, Chrystalla 
Keywords: Drought stress
Antioxidants
Cellular damage
Medicago
Proline
Hydrogen peroxide
Nitric oxide
Reactive oxygen species
Reactive nitrogen species
Issue Date: 2011
Publisher: Landes Bioscience
Source: Plant Signaling & Behavior 6:2, 270-277; February 2011
Abstract: Effects of water stress on plants have been well-documented. However, the combined responses to drought and rewatering and their underlying mechanisms are relatively unknown. The present study attempts to describe spatiotemporal alterations in the physiology and cellular status of Medicago truncatula tissues that result from and subsequently follow a period of moderate water deficit. Physiological processes and cellular damage levels were monitored in roots and leaves by determining lipid peroxidation levels, as well as nitric oxide and hydrogen peroxide content, further supported by stomatal conductance and chlorophyll fluorescence measurements in leaves. During water stress, cells in both organs displayed increased damage levels and reactive oxygen and nitrogen species content, while leaves showed reduced stomatal conductance. Furthermore, both tissues demonstrated increased proline content. Upon rewatering, plants recovered displaying readings similar to pre-stress control conditions. Furthermore, molecular analysis of antioxidant gene expression by quantitative real-time RT-PCR revealed differential spatiotemporal regulation in a number of genes examined (including catalase, cytosolic ascorbate peroxidase, copper/zinc and iron superoxide dismutase and alternative oxidase). Overall, M. truncatula plants demonstrated increased sensitivity to drought-induced oxidative damage; however, this was reversed following rewatering indicating a great elasticity in the plant's capacity to cope with free oxygen radicals.
URI: http://ktisis.cut.ac.cy/handle/10488/4804
DOI: 10.4161/psb.6.2.14633
Rights: ©2011 Landes Bioscience
Appears in Collections:Άρθρα/Articles

Show full item record

SCOPUSTM   
Citations 5

51
checked on Aug 18, 2017

Page view(s)

22
Last Week
1
Last month
3
checked on Aug 19, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.