Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/114
Title: Transcriptome analysis approaches for the isolation of trichome-specific genes from the medicinal plant Cistus creticus subsp. creticus
Authors: Falara, Vasiliki
Fotopoulos, Vasileios ItemCrisRefDisplayStrategy.rp.deleted.icon
Margaritis, Thanasis
Anastasaki, Thalia
Pateraki, Irene
Bozabalidis, Artemios M.
Kafetzopoulos, Dimitris
Demetzos, Costas
Pichersky, Eran
Kanellis, Angelos K. 
Keywords: Cistus creticus subsp. creticus;DNA microarrays;EST analysis;Gene expression;Germacrene B synthase;Trichomes
Category: Biological Sciences
Field: Natural Sciences
Issue Date: 2008
Publisher: Springer Netherlands
Source: Plant Molecular Biology, Vol. 68, Nο 6, 2008, pp.633-651
Abstract: Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from different developmental stages revealed that young leaves of 1–2 cm length displayed the highest content of labdane-type diterpenes (80 mg/g fresh weight) whereas trichomes from older leaves (2–3 or 3–4 cm) exhibited gradual decreased concentrations. A cDNA library was constructed enriched in transcripts from trichomes isolated from young leaves, which are characterized by high levels of labdane-type diterpenes. Functional annotation of 2,022 expressed sequence tags (ESTs) from the trichome cDNA library based on homology to A. thaliana genes suggested that 8% of the putative identified sequences were secondary metabolism-related and involved primarily in flavonoid and terpenoid biosynthesis. A significant proportion of the ESTs (38%) displayed no significant similarity to any other DNA deposited in databases, indicating a yet unknown function. Custom DNA microarrays constructed with 1,248 individual clones from the cDNA library facilitated transcriptome comparisons between trichomes and trichome-free tissues. In addition, gene expression studies in various Cistus tissues and organs for one of the genes highlighted as the most differentially expressed by the microarray experiments revealed a putative sesquiterpene synthase with a trichome-specific expression pattern. Full length cDNA isolation and heterologous expression in E. coli followed by biochemical analysis, led to the characterization of the produced protein as germacrene B synthase.
URI: http://ktisis.cut.ac.cy/handle/10488/114
ISSN: 1573-5028 (Online)
DOI: 10.1007/s11103-008-9399-0
Rights: © Springer
Type: Article
Appears in Collections:Άρθρα/Articles

Show full item record

SCOPUSTM   
Citations 10

26
checked on Feb 15, 2019

WEB OF SCIENCETM
Citations 5

26
Last Week
0
Last month
checked on Feb 17, 2019

Page view(s) 50

57
Last Week
1
Last month
2
checked on Feb 18, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.