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Title: Multiple field and glasshouse assessments increase the reliability of linkage mapping of the Vf source of scab resistance in apple
Authors: King, Graham
Alston, Frank H.
Brown, Lance M.
Chevreau, Elisabeth
Evans, Kate M.
Dunemann, Frank
Janse, Jaap D.
Laurens, François N D
Lynn, James R.
Maliepaard, Chris A.
Roche, Paul
Schmidt, Hanna H.
Tartarini, Stefano
Verhaegh, Jac J.
Vrielink, Ria
Manganaris, George A. ItemCrisRefDisplayStrategy.rp.deleted.icon
Keywords: Malus;Apple;Venturia;Scab resistance;Linkage mapping;Cluster analysis
Issue Date: 1998
Publisher: Springer Berlin / Heidelberg
Source: TAG Theoretical and Applied Genetics. Volume 96, Number 5 / April, 1998, pp. 699-708
Abstract: Apple scab, caused by the fungus Venturia inaequalis (Cke.) Wint., is an important disease in commercial apple production. A mapping population of 155 individuals, derived from a cross between the apple varieties ‘Prima’ (resistant)בFiesta’ (susceptible), was scored for response to the disease in replicated field and glasshouse trials throughout Europe. Twenty data sets were selected and cluster analysis was used to form a consensus score for the population fitting a 1 : 1 segregation ratio of resistance:susceptibility. The progeny were scored with molecular markers. A detailed map covering 54 cM of the ‘Prima’ linkage group containing the Vf gene for scab resistance was constructed using 24 molecular markers linked to the resistance gene. One isoenzyme marker (Pgm-1), six RFLP markers and 17 RAPD markers formed a linkage group with the consensus measure of resistance to scab. Four marker bridges were established with the corresponding ‘Fiesta’ linkage group with additional markers (one isozyme, one RFLP, three RAPD and one AFLP). A low chi-square value indicated a good fit of the marker ordering, which was in close agreement with previously reported linkage positions for some of the markers and Vf. Differences were observed in the ability of different scoring methods to resolve susceptible and resistant classes. The results obtained for the consensus classification of resistance to scab for the population may suggest the presence of virulent inocula at some sites, which could overcome the Vf gene for resistance. The consequences of relying on individual scoring occasions for studying Vf scab resistance are discussed in the context of linkage analysis, conventional breeding selection, and marker-assisted selection.
ISSN: 0040-5752 (Print)
1432-2242 (Online)
DOI: 10.1007/s001220050791
Rights: © Springer
Type: Article
Appears in Collections:Άρθρα/Articles

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