Please use this identifier to cite or link to this item: http://ktisis.cut.ac.cy/handle/10488/10268
Title: Biodiversity and ochratoxin A profile of Aspergillus section Nigri populations isolated from wine grapes in Cyprus vineyards
Authors: Pantelides, Iakovos 
Aristeidou, Efi 
Lazari, Maria 
Tsolakidou, Maria Dimitra 
Tsaltas, Dimitrios 
Christofidou, Maria 
Kafouris, Demetris 
Christou, Eftychia 
Ioannou, Nicolas 
Keywords: Aspergillus section Nigri;Meteorological conditions;Ochratoxin A (PubChem CID: 442530);Ochratoxin-A;Wine grapes
Category: Other Agricultural Sciences
Field: Agricultural Sciences
Issue Date: 1-Oct-2017
Publisher: Academic Press
Source: Food Microbiology, Volume 67, 2017, Pages 106-115
metadata.dc.doi: http://dx.doi.org/10.1016/j.fm.2017.06.010
Abstract: The objective of this study was to evaluate the biodiversity of Aspergillus section Nigri populations from Cyprus vineyards by morphological, toxigenic and phylogenetic analysis. Aspergillus section Nigri populations were isolated from grapes of the varieties ‘Maratheftiko’ and ‘Cabernet Sauvignon’ originating from six growing regions of Cyprus during 2010 and 2011 years. The isolation frequency of Aspergillus section Nigri from grape samples was 43.3% and a total of 284 isolates were selected for further analyses based on the macroscopic characteristics of black aspergilli. The isolates were characterized by sequencing analysis of the calmodulin gene in order to identify species responsible for ochratoxin A (OTA) production. The phylogenetic analysis showed that the isolates were grouped in three major clusters. The A. tubingensis cluster included 262 isolates (92.25%), the A. niger cluster included 15 isolates identified as A. niger (5.3%) and 6 isolates identified as A. welwitschiae (2.1%). One isolate was classified as A. carbonarius (0.35%) and was grouped in a cluster together with the reference isolates of A. carbonarius, A. sclerotioniger, A. sclerotiocarbonarius and A. ibericus. All the isolates were evaluated for their ochratoxigenic ability by HPLC coupled with a fluorescence detector (HPLC-FLD) and the positive isolates were re-examined using ultra high-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The Aspergillus carbonarius isolate produced an average quantity of 1436.1 ng OTA/g Czapek Yeast Agar (CYA); From the A. niger strains three isolates (20%) produced OTA and only one isolate from A. welwitschiae (16.7%) was proved ochratoxigenic with toxin production average at 23.9 ng/g and 9.1 ng/g CYA respectively. Grape must samples derived from the collected berries were also analyzed for OTA and none of the samples were found contaminated with the mycotoxin. The results showed that the geographic area and the meteorological conditions had no significant effect on the incidence and the distribution of black aspergilli in this 2-year project. However, absence of rainfall and low humidity during the harvesting period were critical for the low incidence of the ochratoxigenic A. carbonarius on grapes.
URI: http://ktisis.cut.ac.cy/handle/10488/10268
ISSN: 07400020
Rights: © 2017 Elsevier Ltd
Type: Article
Appears in Collections:Άρθρα/Articles

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