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Title: Isozyme variation in Verticillium dahliae isolates from Crete
Authors: Ligoxigakis, Eleftherios K.
Fragkiadakis, Georgios A.
Vakalounakis, Demetrios John
Thanassoulopoulos, K. K.
Manganaris, George A. 
Issue Date: 2002
Publisher: Springer Netherlands
Source: Folia Microbiologica 2002. Volume 47, Number 2 / April, 2002, pp. 167-170
Abstract: Fifteen isolates ofVerticillium dahliae (eight of race1, seven of race2; most from the island of Crete, Greece) were examined for isozyme and molecular variation. Among the isozyme banding patterns (zymograms) of six enzymes that were “activity-stained” after electrophoresis in 9% polyacrylamide gels, differences were observed in diaphorase, α-esterase, peroxidase and superoxide dismutase; 2, 2, 3 and 5 different types of zymograms were recorded, respectively. The zymograms could not be correlated with either race1 or2. However, all six isolates originating from the Oropedio (plateau) are, of Lasithi (Crete) showed an esterase zymogram clearly distinguishable from the other isolates. No differences were observed when staining for acid phosphatase or aspartate aminotransferase (‘glutamic-oxaloacetic transaminase’). Furthermore, electrophoresis of random-amplified polymorphic DNA (RAPD) in 2% agarose gels showed that three race-2 isolates from Oropedio of Lasithi could also be distinguished by the RAPD pattern generated with primer OPA-1. The variation observed possibly represents adaptation ofV. dahliae to the Oropedio environment.
ISSN: 0015-5632 (Print)
1874-9356 (Online)
DOI: 10.1007/BF02817676
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